Blastocoel cell-free DNA, a marker of embryonic quality
نویسندگان
چکیده
منابع مشابه
Cardiomyocyte Marker Expression in Mouse Embryonic Fibroblasts by Cell-Free Cardiomyocyte Extract and Epigenetic Manipulation
Background: The regenerative capacity of the mammalian heart is quite limited. Recent reports have focused on reprogramming mesenchymal stem cells into cardiomyocytes. We investigated whether fibroblasts could transdifferentiate into myocardium.Methods: Mouse embryonic fibroblasts were treated with Trichostatin A (TSA) and 5-Aza-2-Deoxycytidine (5-aza-dC). The treated cells were permeabilized w...
متن کاملCell - free DNA: بیومارکر نوین تشخیص آزمایشگاهی
به انواع مختلف اسیدهای نوکلئیک آزاد در پلاسما شامل قطعات DNA ژنومی،DNA میتوکندریایی، DNA و mRNA های ویروسی و همچنین انواع miRNA اصطلاحا Cell–free nucleic acid گفته می شود. بخش مهمی از این اسیدهای نوکلئیک آزاد در پلاسما را Cell–free DNA (cfDNA) تشکیل می دهد. cfDNA به صورت قطعاتی با وزن مولکولی حدود KB 21-0.18 در سرم یا پلاسمای افراد سالم و افراد مبتلا به بسیاری از بیماری ها قابل تشخیص هستند. ...
متن کاملcardiomyocyte marker expression in mouse embryonic fibroblasts by cell-free cardiomyocyte extract and epigenetic manipulation
background: the regenerative capacity of the mammalian heart is quite limited. recent reports have focused on reprogramming mesenchymal stem cells into cardiomyocytes. we investigated whether fibroblasts could transdifferentiate into myocardium. methods: mouse embryonic fibroblasts were treated with trichostatin a (tsa) and 5-aza-2-deoxycytidine (5-aza-dc). the treated cells were permeabilized ...
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Quality of an oocyte’s cytoplasm is an important factor affecting preand postimplantation development of the future embryo. It can be compromised for example by aging, both maternal (i.e. related to the female's age) and postovulatory (i.e. related to the time between ovulation and fertilization). Recently, we have establish a new non-invasive method to assess cytoplasmic quality of fertilized ...
متن کاملIntroducing a New and Simple Protocol for Capillary Electrophoresis of Cell Free Fetal Double Stranded DNA
Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...
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ژورنال
عنوان ژورنال: Fertility and Sterility
سال: 2017
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2017.07.324